MALDI-TOF: Bruker Autoflex III
This is our MALDI-TOF instrument (matrix assisted laser desorption ionization – time of flight) for open access use. The instrument uses Compass™ 1.4 for Flex-series. Both negative and positive ionization modes can be used on the same spot. This instrument is ideal for biopolymers (proteins, RNA, DNA, oligosaccharides), organometallic salts, and many synthetic polymers. The instrument can be operated in linear mode for larger molecules or in reflectron mode – which gives better resolution for molecules that weigh less than 4 kDa.
GC-MSD: Agilent 5973
This is an open access time-of-flight GC mass spectrometer. Unknown compounds in a mixture can be identified using the NIST library of spectra. This instrument is ideal for quantitating known compounds in complex mixtures. The mass range is limited to 800 Daltons.
GC-TOF: Waters Micromass GCT Premier
This is an open access time-of-flight GC mass spectrometer with exact mass measurement capability. Exact mass capability is very useful for structural elucidation of unknown compounds in a sample and in the confirmation of the identity of known compounds that may not be found in the NIST library. The mass range is limited to 800 Daltons. The Agilent GC inlet makes it possible to identify compounds in fairly complex mixtures.
LC-TOF: Agilent 6210A
Accurate-mass data can be obtained on an Agilent 6210A LC-TOF mass spectrometer in positive or negative ion mode. This instrument is equipped with an Agilent Series 1200 HPLC binary pump, and an Agilent Series 1200 autoinjector. The Agilent 6210A LC-TOF is ideal for obtaining publication quality exact mass data on small molecules. In addition, protein and oligonucleotide molecular weights can be obtained in ESI mode using MaxEnt deconvolution. Because the instrument configuration is changed over constantly, this instrument is not open access; samples should be submitted through NUCore.
LC-MS: Bruker AmaZon SL Ion Trap
This is one of our new ion trap instruments for open-access ESI analysis. The instrument uses Compass Software version 1.3 SR2. Chemists can choose a suitable carrier solvent and negative or positive ionization mode. In addition, this instrument is capable of doing MSn fragmentation which can provide additional confirmation of molecular structures. This instrument is set up for analyzing compounds soluble in less polar solvents (e.g. isopropanol, methylene chloride).
LC-MS: Bruker AmaZon X Ion Trap
Equipped with an Agilent 1200 Series HPLC system, this is our new ion trap instrument for open-access LC-MS analysis. The instrument uses Compass Software version 1.4 and also has MaxEnt deconvolution software. Chemists can choose a suitable mobile phase system and either negative or positive ionization mode. In addition, like the AmaZon SL, this instrument is capable of doing MSn fragmentation which can provide additional confirmation of molecular structures. The AmaZon X is especially useful for determining the molecular weights of proteins and polynucleotides up to a molecular weight of about 50 kDa using the MaxEnt software. This instrument is targeted for analyzing compounds soluble in more polar solvents (e.g. water, methanol, acetonitrile).
Combustion Analysis: Elementar Vario-El Cube
Combustion analysis provides a bulk measurement of compound stoichiometry or quantitative determination or organic content in environemntal samples. The analytical method is based on the complete and instantaneous oxidation of the sample by “flash combustion”, which converts all organic and inorganic substances into combustion products. The resulting combustion gases pass through a reduction furnace and are swept into the chromatographic column by the carrier gas (helium), where they are separated and detected by a thermal conductivity detector (TCD), which gives an output signal proportional to the concentration of the individual components of the mixture.
Sample analysis is performed by staff on a fee for service basis. Request access to the the item and an IMSERC staff member will contact you to get started with your project.