Agilent 6120A LC-ToF Training Manual

 

Before you start:

  1. Check Solvent Bottles and calculate if you have enough to go on. If not inform IMSERC
  2. Check to see whether the default LC column is installed. If not, inform IMSERC
  3. If you are installing your own, ensure that you reinstall the default column or a union, after you finish your work.
  4. Always prime the lines before you start; know how to work with the purge valve; and don’t forget to close the purge valve after you finish priming.

Installing a column:

  1. You will have to practice with your trainer on how to install or exchange columns
  2. Small leaks may not be noticed right away
  3. Always know the back pressure of your column set up
  4. Inform IMSERC right away if you see significant (more than 5 bars) differences

Priming the lines:

  1. Load your method (during training, a method would have been written for you with your name on it.
    1. Do not use any other method; inform IMSERC if you need a method developed
  2. Open the purge valve
  3. Set B to zero%
  4. Change flow rate to 3 mL/min (ensure you did open the purge valve) then click apply or hit enter
  5. After a minute or two, set B to 100% and wait another minute or two
  6. Reload your method and say no to changes question
  7. Flow rate should now be at initial conditions (typically 0.25 mL/min) with the proper A and B flow rates.
  8. Close the purge valve

Working with Agilent MassHunter Workstation Data Acquisition Software

  1. Filling out a Worklist, i.e. the Sample List:
    1. A worklist is always open
    2. If not, stop and inform IMSERC; there could have been a unscheduled reboot
    3. You can append the list you see; we don’t have individual worklists
    4. Do not forget to copy and paste the standby script at the bottom of your list
    5. You need to enter Sample Name, Sample Position, Method Name, Data File Name, Sample Type, and Injection Volume.
      1. Sample Name is whatever that helps you identify your sample. Avoid special characters
      2. Sample Position is formatted as Rack Position (P1 front, P2 back) and vial position, e.g. P1-A1.
  • The front row is A, then B, etc. Row F is in the back.
  1. A1 is on the right-front and indexed through A9 going left – so A1-A9, B1-B9, …, F1-F9.
  2. Example — P1-F8 is the front Rack, row F, and 8th position, counting from the right.
  3. Example — Back Rack is called P2.   P2-C3 is the back rack, row C, 3rd position counting from the right.
  1. Copying another line may save a little time
  2. To copy a line, highlight that line by left clicking the worklist row number (far left), hover the cursor on the highlighted area, right click, then left click on Copy Row(s) option. Now highlight the line below which you want to paste the copied line, right click, and select Add Copied Row(s).  This will add the new line below the highlighted line.  To copy above it, click on Insert Copied Row(s).
  3. For selecting methods, click on the cell in Methods column, a drop down arrow becomes visible. Click on the arrow and you will see a short list of recently used methods.  If your method is not there, take the scroll bar to the bottom of the list and click on “other….” Option.  This brings up the Select Method window.  Select your method and click OK.
  4. Caution: Do not move Methods to your folder.  All methods have to be in “D:\MassHunter\methods” directory.
  5. To name your data file, select the data file cell, then click on the three dots icon on the right, navigate to your folder, then write the file name in the box at the bottom, labeled File.
  6. If you want to create a subdirectory in your folder, you can do it by creating it in file explorer first before modifying the worklist or modify the Data file name. Create the file name in your directory, click on okay, and then place the cursor in the middle of the line where you want to place the subdirectory, and type in the name of your subdirectory.
  7. Leave the sample type as Sample. If you are doing quantitation, you will learn about sample types in a separate training session.
  8. Injection volumes are typically 20 uL or less for the default columns we use. Smaller injection volumes give you better chromatography.
  9. Once you have modified the work list and included all your samples (and blanks), place a check mark in the square box to the left of each line you want to execute. Your last line needs to be the standby script.  You can copy and paste this from the worklist.  Look for a gray line starting with Script: SCP_InstrumentStandby.
  10. Now click on the Start Worklist Run icon at the top.