Purity analysis of sample using LC/UV/MS or GCMS

GCMS

An Agilent GCMSD (low resolution, single quadrupole mass spectrometer) fitted with a Headspace module is used to perform trace level analysis of residual solvents from the synthesis process.  Both polar and non-polar GC columns can be used. Unknown compounds in a mixture can be identified using the NIST library of spectra.  This instrument is ideal for quantitating known compounds in a complex mixtures. The mass range is limited to 800 Daltons.

The requester will provide a list of solvents and approximately 15 mg of solid sample for this analysis.

Typical data

 

Peak Purity – LUM

LUM is short for tandem Liquid Chromatography – UV – Mass Spectrometry.  The system allows measuring the concentration of each component of a mixture through a UV detector and the corresponding accurate mass by the high resolution time of flight mass spectrometer.  The Agilent 6210A LC-TOF is ideal for obtaining publication quality exact mass data on small molecules.  In addition, protein and oligonucleotide molecular weights can be obtained in ESI mode using Max Entropy deconvolution.

Typical Data

BioPolymer Molecular Weight Determination

An Agilent 6210A Time-of-Flight LCMS system (resolving power of ~10,000) is used to perform an online sample clean up by liquid chromatography followed by electrospray ionization, high resolution mass spectrometric analysis.  The complexity of the raw data (due to significant contribution from adjacent molecular isotopes under each one of the charge state ion envelope) makes it nearly impossible to manually interpret the data.  Maximum Entropy algorithm is utilized to deconvolute the data into a simple neutral spectrum where each unique molecule is assigned a single Mr.  Relatively small biopolymers of up to 150 kDa has been successfully analyzed.

Typical Data

Below are spectra of raw data, deconvoluted data, and a narrow spectrum showing potential modifications to the macromolecule.