Before NMR Training
Follow the instructions at: http://imserc.facilities.northwestern.edu/about/getting-started/ to create accounts and obtain access to the labs. How to establish your group affiliation for remote data access is at the end of the video.
Step 1: Get certified
Before undergoing training, every user must complete a certification test to demonstrate their knowledge of NMR operation and IMSERC policies. We provide a study guide called “NMR Basics” to help you pass this test. BEFORE coming to your first training session, please download the NMR Basics manual and complete the online quiz at: http://imserctraining.northwestern.edu/NMRQuiz/multiscreen.html . Once you pass the quiz, you will receive email confirmation which tells you where/when the regular training sessions are. Then you can let IMSERC staff member know if you are coming to a regular training session or need arrange a different time due to schedule conflict.
Step 2: Basic NMR Training
I. Agilent (automation for Au400, manual / study queue operation for I500, Hg400, F500 and HCN600): Dr. Yongbo Zhang holds a regular training session every Wednesday morning at 10:00 AM (about one hour). This training will cover Au400, I500, Hg400, F500, and HCN600. You do not need to apply separately for training on each of these instruments.
Read this brief VnmrJ manual before you come. Please confirm your attendance. Please bring your completed certification test, and come dressed appropriately for the lab (glasses or safety glasses, etc.). After training session and checking your written test, you will be authorized using NMR-Au400, I500, and maybe the other three depending on your experience. With more practicing, you will be granted access to Hg400, F500 and HCN600 upon request without further training. If your schedule doesn’t fit our regular training time, you may talk to NMR staff to arrange a special session for you.
II. Bruker (automation for Ag500): Dr. Yuyang Wu holds a regular training session every Tuesday at 9:00 AM in Silverman B530 (up to 1 hour). Please confirm your attendance. Please bring your completed certification test, and come dressed appropriately for the lab (glasses or safety glasses, etc.). If your schedule doesn’t fit our regular training time, you may talk to NMR staff to arrange a special session for you.
III. Bruker (manual operation for A600) Dr. Yuyang Wu holds a regular training session every other Thursday at 1:00 PM (up to 3 hours). You must have some experience with manual operation of NMR spectrometers before getting trained on A600. Training sessions include VT operation and experiment optimization, and they may be customized to suit your particular needs (e.g., how to conduct DOSY experiments). Please apply for A600 training in NUCore and contact Dr. Wu for availability.
Step 3: Advanced Training (upon request)
I. VT operation: Once you’ve been authorized to use the NMR systems, you may wish to conduct NMR experiments with different sample temperatures. IMSERC staff is the ONLY personnel who may train you how to regulate sample temperature – NO LABMATES! To apply, simply reserve your experiment time and coordinate with Yuyang or Yongbo to make sure he is available when you start. This should take only ~15 minutes.
II. DOSY: You can run DOSY experiments on A600, F500, and HCN600. The training is available on request. Here is a short introduction of DOSY. The instruction manuals for both VnmrJ and Topspin are also available here and here.
III. Selective 1D/2D: To simplify your NMR spectrum, the selective 1D & 2D experiments are the powerful tools that synthetic chemists can employ. Here is a short video about selective 1D zTOCSY on Amikacin sulfate.
IV. Solids NMR: IMSERC’s 400 MHz Varian VNMRS Solids NMR spectrometer is powerful and very popular with researchers who work on samples for which dissolution would not be appropriate or possible. It can run many hetero-nuclei, such as C13, Al27, Si29, P31, Sn119, etc. Dr. Yuyang Wu provides training as needed. Please apply for Solids NMR training in NUCore and contact Dr. Wu for availability
V. HCN600 NMR: This triple resonance (HCN) NMR spectrometer is designed for bio-macromolecular samples and for long term data collecting. It’s equipped with a HCN Cold probe, and has the best H1 sensitivity (S/N=4300:1). Please apply for this NMR in NUCore and contactDr. Yongbo Zhang for training.
VI. Data processing and analysis with Mnova: You can download Mnova from Mestralab. NU has site license available from the “public” share at IMSERC file server. Use data access manual to map this share. Here is the training manual for Mnova. A shorter version is also available.
We take requests!