Mass Spectrometry FAQ:
- How do I fill out the form?
- My samples are air/temperature/light/time-sensitive, how should I handle them?
- What happens to my samples when you are done with the experiment?
- I left my sample in the queue and haven’t heard anything. What’s going on?
- How do I decide what instrument to use for my sample?
- How do I make MNOVA work for Bruker MALDI-ToF or AmaZon-X and -SL data?
1. How do I fill out the form for LC-TOF analysis? Log on to NUCORE and select “MS Sample Submission” (https://nucore.northwestern.edu/facilities/imserc/services/ms_sample_submission ) from the side-menu. Complete the form, save your cart and then print the form.
2. My samples are air/temperature/light/time-sensitive, how should I handle them? If your samples will decompose rapidly under ambient conditions, please leave them in a safe place in your lab. Fill out a sample submission form and leave it in the queue. You must also contact Saman or Dan to arrange a time to bring your samples to the lab for analysis.
3. What happens to my LC-TOF samples when you are done with the experiment? If you know you will need your sample returned, write a comment on the submission form and we will save what material we can. Unused material is deposited in the finished sample area on the bench by the queue and may be picked up at any time. We empty the boxes approximately every two weeks, so be sure to retrieve your sample as soon as possible after the experiment.
4. I left my LC-TOF sample in the queue and haven’t heard anything. What’s going on? The most likely explanation is that your sample is still waiting its turn in the queue. If you are concerned, feel free to e-mail us, or come by the lab. Most samples are analyzed within one week.
5. How do you decide which open access instrument to use for my sample?We have multiple instruments available for open access use. The best instrument to use will depend on the molecular weight, functional groups present in the molecule, and the complexity of the sample. See our instrument descriptions for details on each instrument.
6. How do I make MNOVA work for Bruker MALDI-ToF or AmaZon-X and -SL data? CompassXtract executable file needs to be on your hard drive for MNOVA to work with Bruker MALDI-ToF file types. See the detailed step by step instructions in this link. For AmaZon-X and -SL, MNOVA is compatible with PC only. If you are having issues with MNOVA, open the file in MNOVA at the instrument PC, display the data as you would like to view it, then save the MNOVA file in your data directory. You can now open the MVONA file with any operating system.