Ligand screening

NMR is a powerful technique to study macromolecule (protein, DNA, and RNA) ligand binding. This can be done in two ways. The first is to isotopically enrich the macromolecule with 13C and/or 15N and collect the HSQC NMR spectrum upon binding of ligands. It’s called structure-activity relationship (SAR) by NMR. The second one is to monitor ligainds with NMR upon binding of macromolecule. There are a few techniques in this category. Some of most popular ones are saturation transfer difference (STD) NMR and Water-Ligand Observed via Gradient SpectroscopY (WaterLOGSY). You can run all these experiments on HCN600 with BioPack after trained by staff. The NMR staff also provide service on SAR by NMR,  STD NMR, and WaterLOGSY. Please click to start a project.